Viral Vector Core Facility (Hollings Cancer Center, Microbiology and Immunology) (rev. 10/07)
http://hcc.musc.edu/research/sharedresources/index.htm
Director: Jian-yun Dong, MD, PhD; Associate Director: Semyon Rubinchik, PhD
The facility provides design and construction of various viral vectors for members of the MUSC research community as well as outside academic and corporate collaborators. It provides a wide array of services, such as creating recombinant adenovirus and producing high-titer viral stocks, producing high titers of helper virus-free AAV vectors, establishing retroviral/lentiviral vector-producer cells, generating and concentrating viral stocks for transduction, and providing quality control and standardized vectors for gene transfer studies. In addition to standard vectors, the facility develops adenoviral vectors with custom features, such as tissue-specific promoters, drug-regulated expression systems, and siRNA expression systems. The facility is located in the Hollings Cancer Center, and is supported by the Hollings Cancer Center, an NCI R24 research resources award, and the Department of Microbiology & Immunology. The facility is staffed by a full-time molecular biologist who works with individual investigators to optimize production and purification of viral vectors.